Gomez and her band The Scene performed for the organisation last year, and they took to the stage again to raise more cash for the fund, reporeted Femalefirst online.
Bieber was on hand to offer his partner moral support, while soccer ace Beckham took his sons to the gig.
Gomez later took to her Facebook to thank her fans for their support.
“Thank You to all who came out to the show last night at House of Blues on Sunset. That is one of the shows I look forward to the most and cannot wait for next year’s show. I don’t have an exact number but together we all raised over 200,000 dollars to benefit Unicef,” she posted.
“We’re helping to save the lives of children all over the world,” she added.